Radiomics-Based Visualization and Quantitative Validation of IDH1 Heterogeneity in Gliomas

• ClinicalTrials.gov Identifier: NCT05969691
• Recruitment Status: Active, not recruiting
• First Posted: August 1, 2023
• Last Update Posted: August 8, 2023
• Sponsor: Mingge LLC
• Collaborators: Huashan Hospital; Fudan University
• Information provided by (Responsible Party): Mingge LLC

Study Description

Brief Summary:

The goal of this clinical trail is to non-invasively visualise and quantitatively validate an radiomics model of genetic heterogeneity in adult patients with diffuse glioma to help clinicians better guide surgical resection and treatment options. It aims to answer are:

1. To overcome the limitations of the existing genetic diagnostic process in terms of equipment and technology requirements, high costs and long timelines, and to enable quantitative studies of isocitrate dehydrogenase 1 (IDH1) mutations, thus allowing refined patient stratification and further exploration of the role of molecular markers in improving patient prognosis.
2. To achieve non-invasive diagnosis of gene mutations within tumours by taking advantage of artificial intelligence and medical images, and to test the clinical feasibility of the model through typical target puncture, gene sequencing and quantitative gene expression analysis.

Participants will read an informed consent agreement before surgery and voluntarily decide whether or not to join the experimental group. They will undergo preoperative magnetic resonance imaging, intraoperative brain puncture of typical tumour sites, and postoperative genotype identification. Their imaging data, genotype data, clinical history data, and pathology data will be used for the experimental study.

Condition or disease	Intervention/treatment	Phase
Glioma, Malignant; Computer-Assisted	Diagnostic Test: Validation of IDH1 mutations from the radiomics model	Not Applicable

Detailed Description:

BACKGROUND

The WHO 2016 officially introduced molecular markers into the pathological diagnosis of gliomas, marking a step into the era of molecular diagnosis of gliomas. Among them, isocitrate dehydrogenase 1 (IDH1) mutation is considered to be the 'backbone' in the development of gliomas, and affects the treatment plan and prognosis of patients. However, the clinical use of this molecular biomarker is still controversial, which is rooted in the lack of quantitative studies on IDH1 mutations. The spatial heterogeneity of gliomas has been demonstrated in existing studies, i.e., tumor tissues in different parts of the same glioma belong to different genetic subtypes. This implies that IDH1-mutant tumors do not indicate the presence of mutations in all tumor cells, thus further exacerbating the problems in clinical genetic diagnosis.

OBJECTIVE

To quantify gene mutations in tumours, we plan to use radiomics model with artificial intelligence and clinical big data, and verify its accuracy by tissue puncture. In this way, we can overcome the challenges of multisite sampling and second-generation sequencing, such as high equipment and technology requirements, high cost and long time, and thus theoretically realise the visualisation and quantification of genetic heterogeneity within gliomas.

PROCESS

Participants will read an informed consent agreement before surgery and voluntarily decide whether or not to join the experimental group.

1. Modelling of visualisation of genetic heterogeneity

   Before surgery, participants first Routine imaging and the resulting images will be used to build a radiomics model. The model will non-invasively predict IDH1 mutations in gliomas.

2. Typical site puncture

   After the enrolled participants were anaesthetised and craniotomised, clinicians selected typical tumor sites for puncture based on the model outputs.

3. Histopathological diagnosis

   The specimen from the same puncture site is divided into two parts, and the first part is routinely formalin-fixed for paraffin embedding and finally H&E-stained sections. The pathologist first reads the H&E sections and makes a histological diagnosis, describing the pathological morphology and characteristics, especially the tumor cell content and distribution.

4. IDH1 single nucleotide sequencing

   Another part of the sample is used for liquid nitrogen preservation. The Qiagen DNA/RNA Extraction Kit is used to extract DNA from the liquid nitrogen preserved tumour tissue, which is purified and subjected to the IDH1 polymerase chain reaction (PCR). The PCR product is purified and subjected to sequencing, and the sequencing product is detected on an ABI 7200 sequencer to determine whether IDH1 is mutated or not.

5. Mass spectrometry analysis of 2-Hydroxyglutarate (2-HG) expression levels

   The presence and expression of 2-HG in glioma samples is detected and analysed by mass spectrometry.

6. Validation of the radiomics-based IDH1 mutation prediction model. The 2-HG detection results were numerically compared with the model results.

This is a single centre validation study. Compared with the routine glioma surgical procedure, this study adds intraoperative tumor-typical sits puncture to validate the predictive accuracy of the radiomics model and collects corresponding MRI images, tumour histology diagnosis, molecular pathology diagnosis. The radiomics model is built based on preoperative clinical data and is a non-invasive and rapid tool for quantitative analysis and visualisation of tumor genes.

Study Design

• Study Type: Interventional (Clinical Trial)
• Actual Enrollment: 18 participants
• Allocation: N/A
• Intervention Model: Single Group Assignment
• Masking: None (Open Label)
• Primary Purpose: Other
• Official Title: Radiomics-Based Visualization and Quantitative Validation of Isocitrate Dehydrogenase 1 Heterogeneity in Gliomas
• Actual Study Start Date: March 15, 2019
• Estimated Primary Completion Date: December 15, 2025
• Estimated Study Completion Date: December 15, 2026

Arms and Interventions

Arm

Intervention/treatment

Experimental: Validation of IDH1 mutations from the radiomics model; In this experiment, we validate the accuracy of radiomics model for IDH1 prediction by puncturing typical targets of gliomas, gene sequencing and quantitative gene analysis.

Diagnostic Test: Validation of IDH1 mutations from the radiomics model; During surgery, clinicians will select several typical target sites for puncture based on the preoperative model outputs. After surgery, the acquired tumour tissues will be subjected to histological diagnosis and pathological diagnosis by professional pathologists, and the results will be compared with the model output to verify its accuracy.

Outcome Measures

Primary Outcome Measures :

1. accuracy [ Time Frame: End of study (24 weeks) ]
   The ratio of correct model predictions to all predictions is calculated by comparing the results with the puncture results.

Eligibility Criteria

• Ages Eligible for Study: 18 Years to 80 Years (Adult, Older Adult)
• Sexes Eligible for Study: All
• Accepts Healthy Volunteers: No

Criteria

Inclusion Criteria:

• Patients aged 18-70 years, male or female;
• Patients with complete preoperative magnetic resonance imaging and were initially diagnosed with glioma based on the magnetic resonance imaging results;
• Patients who were proposed for craniectomy without contraindications to surgery and who had signed an informed consent form by themselves or their proxy;
• Patients with confirmed diagnosis of glioma by postoperative pathological diagnosis.

Exclusion Criteria:

• Patients to be admitted for a simple puncture biopsy;
• Patients with a combination of serious medical conditions and those who cannot tolerate surgery;
• Patients with a bleeding tendency or abnormal coagulation function, or those who have not discontinued long-term use of anticoagulants such as aspirin and clopidogrel;
• Pregnant and breastfeeding women;
• Patients participate in other clinical trials during the same period.

Contacts and Locations

Locations

China, Shanghai

Huashan Hospital, Fudan University

Shanghai, Shanghai, China, 200433

Sponsors and Collaborators

Mingge LLC

Huashan Hospital

Fudan University

Investigators

• Study Chair: Ying Mao, DM; Huashan Hospital
• Study Director: Zhifeng Shi, DM; Huashan Hospital
• Study Director: Jinhua Yu, DE; Fudan University
• Principal Investigator: Zengxin Qi, DM; Huashan Hospital

More Information

• Responsible Party: Mingge LLC
• ClinicalTrials.gov Identifier: NCT05969691
• Other Study ID Numbers: MINGGE-SW-00003-V1-01
• First Posted: August 1, 2023
• Last Update Posted: August 8, 2023
• Last Verified: August 2023

Individual Participant Data (IPD) Sharing Statement:

• Plan to Share IPD: No
• Plan Description: All patient information collected by the Huashan Hospital will be kept strictly confidential. We will share the results and findings of the study with other researchers.

• Studies a U.S. FDA-regulated Drug Product: No
• Studies a U.S. FDA-regulated Device Product: No

Keywords provided by Mingge LLC:

Genetic Heterogeneity; Gliomas; Computer-Assisted Diagnosis

Additional relevant MeSH terms:

Glioma; Neoplasms, Neuroepithelial; Neuroectodermal Tumors; Neoplasms, Germ Cell and Embryonal; Neoplasms by Histologic Type; Neoplasms; Neoplasms, Glandular and Epithelial; Neoplasms, Nerve Tissue