Relation Between NAFLD and BM DENSITY

• ClinicalTrials.gov Identifier: NCT06038253
• Recruitment Status: Not yet recruiting
• First Posted: September 14, 2023
• Last Update Posted: September 15, 2023
• Sponsor: Assiut University
• Information provided by (Responsible Party): Mina maged helmy habib, Assiut University

Study Description

Brief Summary:

In general NAFLD is a common denominal for a broad spectrum of damage to the liver, which can be due to hepatocyte injury, inflammatory processes and fibrosis. This is normally seen on liver biopsy and can range from milder forms (steatosis) to the more severe forms (non-alcoholic steatohepatitis (NASH), advanced fibrosis, cirrhosis and liver failure). In these patients, advanced fibrosis is the major predictor of morbidity and liver-related mortality, and an accurate diagnosis of NASH and NAFLD is mandatory .

NAFLD is closely associated with metabolic disorders, including central obesity, dyslipidaemia, hypertension, hyperglycaemia and persistent abnormalities of liver function tests. NAFLD was shown to be connected with diseases that are usually not dependent on obesity, such as sarcopenia and osteoporosis

Target of the study :

1. Clarify predictive value of fibroscan and u/s in diagnosis of NAFLD.
2. Estimate the metabolic effect of NAFLD on bone density
3. Estimate the correlation between obesity , NAFLD and BMD

Condition or disease	Intervention/treatment
NAFLD; Osteoporosis	Device: Fibroscan

Detailed Description:

INTRODUCTION:

In general, the prevalence of NAFLD has increased over the last 20 years. The Middle East and South America have the highest NAFLD prevalence at 31% and 32% respectively with the lowest prevalence in Africa at 13.5% .

NAFLD is closely associated with metabolic disorders, including central obesity, dyslipidaemia, hypertension, hyperglycaemia and persistent abnormalities of liver function tests. NAFLD was shown to be connected with diseases that are usually not dependent on obesity, such as sarcopenia and osteoporosis In general NAFLD is a common denominal for a broad spectrum of damage to the liver, which can be due to hepatocyte injury, inflammatory processes and fibrosis. This is normally seen on liver biopsy and can range from milder forms (steatosis) to the more severe forms (non-alcoholic steatohepatitis (NASH), advanced fibrosis, cirrhosis and liver failure). In these patients, advanced fibrosis is the major predictor of morbidity and liver-related mortality, and an accurate diagnosis of NASH and NAFLD is mandatory .

Osteoporosis is a group of bone diseases with various causes, including general factors related to aging, obesity and sex steroid deficiency, as well as specific risk factors such as the use of glucocorticoids, reduced bone quality, and disruption of microarchitectural integrity. In most cases of osteoporosis, the reduction in bone tissue is mainly due to increased bone resorption. Osteoporosis is characterized by low bone mineral density (BMD), bone pain and easy fracture.Osteoporosis is a silent disease until fractures occur with increasing frequency, which can cause important problems and even death. In industrialized countries, 9%-38% of women and 1%-8% of men >50 years suffer from osteoporosis.

Therefore, osteoporosis is not only harmful to health but also increases the financial burden of the impacted countries.

Many studies have demonstrated that NAFLD is associated with low BMD and osteoporosis.

Liver is the source of many proteins and is the regulator of several pathways involving bone metabolism; one of the most well-known of all is vitamin D metabolism pathway. Considering the role of liver in bone metabolism, the association between NAFLD and bone abnormalities is not surprising especially with substantial supporting evidences in recent years .

Besides its role in the calcium and bone metabolism, vitamin D may also exert pleiotropic effects in many tissues. NAFLD patients were reported to have a marked reduction in serum 25(OH) vitamin D when compared with control Liver biopsy remains the current gold standard for diagnosis of NAFLD, despite limitations regarding sampling variability, invasive nature, and high cost. However, numerous non-invasive biomarkers, including mainly serum markers or imaging modalities, intend to detect the presence of steatosis, NASH or advanced fibrosis. To date, ultrasound is suggested as first-line screening tool for defining steatosis in a selected population, while diagnosis of NAFLD requires exclusion of other chronic liver disease etiology or other steatosis causes and exclusion of alcohol intake by history .

Fibro scan becomes an evidence based , transient elastography instrument For noninvasive evaluation of liver steatosis and fibrosis , fibroscan is becoming an increasing important modality in NAFLD practice , as it was recently used to identify patients eligible for NAFLD related clinical trials

Target of the study :

1. Clarify predictive value of fibroscan and u/s in diagnosis of NAFLD.
2. Estimate the metabolic effect of NAFLD on bone density
3. Estimate the correlation between obesity , NAFLD and BMD

STUDY DESIGN :

CROSS- SECTIONAL OBSERVATIONAL STUDY

Patients and methods :

1. Patients presented to obesity clinic for follow up
2. Patients presented to git clinic
3. Patients presented to fibroscan clinic for evaluation the degree of liver fibrosis and steatosis will be included in the study
4. Patients presented at radiology department for abd U.S and diagnosed accidentally fatty liver .

INCLUSION CRITERIA

1. Patients above the age of 18 y.o and premenopausal females
2. Patients showing any degree of fibrosis and steatosis .
3. Patients suffers from obesity and its complications
4. Patients with hyperlipidemia and diabetes mellitus .
5. NAFLD patients were diagnosed with an ultrasound examination or fibroscan or pathological examination to make a clear and definite diagnosis,
6. all the obese participants according to body mass index (BMI)
7. BMD was measured by dual energy X-ray absorptiometry.

Exclusion criteria :

1. Patients less than 18 y.o
2. Patients known alcohol abuser of ≥30 g/day in men or≥20 g/day in women.
3. Patients with chronic liver disease or hepatocellular carcinoma .
4. Subjects with suspected or proven any other liver disease other than NAFLD as (viral hepatitis, drug-induced liver injury, autoimmune liver disease, Wilson's disease or primary biliary cholangitis).
5. none of the subjects followed specific diets or therapeutic treatments that could influence BMD or liver function.
6. Patients with conditions known to affect bone metabolism, such as kidney, thyroid or parathyroid diseases, bone tumors
7. (iv) Postmenopausal females to avoid the increased risk of osteoporosis in those patients due to hormonal changes.

Study Design

• Study Type: Observational
• Estimated Enrollment: 100 participants
• Observational Model: Case-Control
• Time Perspective: Retrospective
• Official Title: Relation Between Non- Alcoholic Fatty Liver Disease and Bone Mineral Density in Egyptian Adults
• Estimated Study Start Date: October 1, 2023
• Estimated Primary Completion Date: October 1, 2026
• Estimated Study Completion Date: December 1, 2026

Groups and Cohorts

Intervention Details:

• Device: Fibroscan
  Fibroscan is device to detect degree of liver fibrosis in NAFLD patients Dexa scan device detect degree of osteoporosis in NAFLD patients
  Other Name: Dexa scan

Outcome Measures

Primary Outcome Measures :

1. Level of vit D level in serum in NAFLD patients [ Time Frame: 1 year ]
   To study level of vit D in NAFLD patients and its relation to disease severity
2. Degree of osteoporosis in NAFLD patients [ Time Frame: 1 year ]
   Using dexa scan to detect level of osteoporosis in NAFLD patients
3. Level of parathyroid hormone level in NAFLD PATIENTS [ Time Frame: 1 year ]
   Level of parathyroid hormone level in NAFLD PATIENTS and its relation to disease severity

Eligibility Criteria

• Ages Eligible for Study: 18 Years to 50 Years (Adult)
• Sexes Eligible for Study: All
• Sampling Method: Non-Probability Sample

Study Population

1. Patients presented to obesity clinic for follow up
2. Patients presented to git clinic at Alrajhi hospital
3. Patients presented to fibroscan clinic for evaluation the degree of liver fibrosis and steatosis will be included in the study
4. Patients presented at radiology department for abd U.S and diagnosed accidentally fatty liver .

Criteria

Inclusion Criteria:

- INCLUSION CRITERIA

1. Patients above the age of 18 y.o and premenopausal females
2. Patients showing any degree of fibrosis and steatosis .
3. Patients suffers from obesity and its complications
4. Patients with hyperlipidemia and DM
5. NAFLD patients were diagnosed with an ultrasound examination or fibroscan or pathological examination to make a clear and definite diagnosis,
6. all the obese participants according to body mass index (BMI)
7. BMD was measured by dual energy X-ray absorptiometry.

Exclusion Criteria:

• 1- Patients less than 18 y.o 2- Patients known alcohol abuser of ≥30 g/day in men or≥20 g/day in women. 3- Patients with chronic liver disease or HCC. 4- Subjects with suspected or proven any other liver disease other than NAFLD as (viral hepatitis, drug-induced liver injury, autoimmune liver disease, Wilson's disease or primary biliary cholangitis).

  5- none of the subjects followed specific diets or therapeutic treatments that could influence BMD or liver function.

  6- Patients with conditions known to affect bone metabolism, such as kidney, thyroid or parathyroid diseases, bone tumors 7- (iv) Postmenopausal females to avoid the increased risk of osteoporosis in those patients due to hormonal changes.

Contacts and Locations

Contacts

Contact: Mina Maged helmy habib, Reident of internal medecine; +201225760412; mon.maged@yahoo.com

Contact: Ahmed Mohammed mostafa ashmawy, Assistant professot; +201005679880; dr.ashmawy@yahoo.com

Sponsors and Collaborators

Assiut University

Investigators

• Study Director: Ghada Abdelrahman alkhateeb, Professor; Assiut University
• Study Director: Ahmed Mohammed mostafa ashmawy, Assistant professor; Assiut University

More Information

Publications of Results:

Loomba R, Sanyal AJ. The global NAFLD epidemic. Nat Rev Gastroenterol Hepatol. 2013 Nov;10(11):686-90. doi: 10.1038/nrgastro.2013.171. Epub 2013 Sep 17.

Eshraghian A. Bone metabolism in non-alcoholic fatty liver disease: vitamin D status and bone mineral density. Minerva Endocrinol. 2017 Jun;42(2):164-172. doi: 10.23736/S0391-1977.16.02587-6. Epub 2016 Dec 14.

Poggiogalle E, Donini LM, Lenzi A, Chiesa C, Pacifico L. Non-alcoholic fatty liver disease connections with fat-free tissues: A focus on bone and skeletal muscle. World J Gastroenterol. 2017 Mar 14;23(10):1747-1757. doi: 10.3748/wjg.v23.i10.1747.

Other Publications:

Sasso M, Audiere S, Kemgang A, Gaouar F, Corpechot C, Chazouilleres O, Fournier C, Golsztejn O, Prince S, Menu Y, Sandrin L, Miette V. Liver Steatosis Assessed by Controlled Attenuation Parameter (CAP) Measured with the XL Probe of the FibroScan: A Pilot Study Assessing Diagnostic Accuracy. Ultrasound Med Biol. 2016 Jan;42(1):92-103. doi: 10.1016/j.ultrasmedbio.2015.08.008. Epub 2015 Sep 19.

Ballestri S, Zona S, Targher G, Romagnoli D, Baldelli E, Nascimbeni F, Roverato A, Guaraldi G, Lonardo A. Nonalcoholic fatty liver disease is associated with an almost twofold increased risk of incident type 2 diabetes and metabolic syndrome. Evidence from a systematic review and meta-analysis. J Gastroenterol Hepatol. 2016 May;31(5):936-44. doi: 10.1111/jgh.13264.

• Responsible Party: Mina maged helmy habib, Resident doctor, Assiut University
• ClinicalTrials.gov Identifier: NCT06038253
• Other Study ID Numbers: NAFLD and bone mineral density
• First Posted: September 14, 2023
• Last Update Posted: September 15, 2023
• Last Verified: September 2023

Individual Participant Data (IPD) Sharing Statement:

• Plan to Share IPD: Undecided

• Studies a U.S. FDA-regulated Drug Product: No
• Studies a U.S. FDA-regulated Device Product: No

Additional relevant MeSH terms:

Osteoporosis; Bone Diseases, Metabolic; Bone Diseases; Musculoskeletal Diseases; Metabolic Diseases