Phase I Safety Study of B/HPIV3/S-6P Vaccine Via Nasal Spray in Adults

• ClinicalTrials.gov Identifier: NCT06026514
• Recruitment Status: Recruiting
• First Posted: September 7, 2023
• Last Update Posted: November 8, 2023
• Sponsor: National Institute of Allergy and Infectious Diseases (NIAID)
• Collaborators: Johns Hopkins Bloomberg School of Public Health; National Institutes of Health (NIH)
• Information provided by (Responsible Party): National Institute of Allergy and Infectious Diseases (NIAID)

Tracking Information

• First Submitted Date: August 30, 2023
• First Posted Date: September 7, 2023
• Last Update Posted Date: November 8, 2023
• Actual Study Start Date: September 18, 2023
• Estimated Primary Completion Date: November 2024 (Final data collection date for primary outcome measure)

Current Primary Outcome Measures (submitted: August 30, 2023)

• To determine the frequency of vaccine-related solicited adverse events (AEs) [ Time Frame: During study days 0 to 28 and 56 to 84 (28 days after each dose) ]
  Summary and line listing of individual clinical solicited of the frequency of solicited AEs
• To determine the frequency of vaccine-related unsolicited AEs [ Time Frame: During study days 0 to 28 and 56 to 84 (28 days after each dose) ]
  Summary and line listing of individual clinical solicited of the frequency of unsolicited AEs
• To determine the frequency of vaccine-related lower respiratory illness [ Time Frame: During study days 0 to 28 and 56 to 84 (28 days after each dose) ]
  Summary and line listing of individual clinical solicited of the frequency of vaccine-related lower respiratory illness
• Vaccine virus shedding on 1 or more days [ Time Frame: On Days 4, 7, and 10, and 60, 63, and 66 ]
  As assessed by culture, or rRT-PCR
• Evidence of a >4-fold rise in HPIV3 antibody titers [ Time Frame: When comparing pre-vaccination titers day 29, 56 or 90 ]
  Using PRNT60 or ELISA

• Original Primary Outcome Measures: Same as current

Current Secondary Outcome Measures (submitted: August 30, 2023)

Rise in serum neutralizing antibody titer to SARS-CoV-2 S protein [ Time Frame: On days 63, 70, 84, 180, 360 ]

Will be measured by ELISA and neutralization assay

• Original Secondary Outcome Measures: Same as current
• Current Other Pre-specified Outcome Measures: Not Provided
• Original Other Pre-specified Outcome Measures: Not Provided

Descriptive Information

• Brief Title: Phase I Safety Study of B/HPIV3/S-6P Vaccine Via Nasal Spray in Adults
• Official Title: A Phase I Study of the Safety and Immunogenicity of the Recombinant, Live-Attenuated, Bovine/Human Parainfluenza Virus Vector Vaccine Expressing the 6P-Prefusion-Stabilized Version of the SARS-CoV-2 Spike Protein, Administered in Two Sequential Doses as Nasal Spray to Adults 18 to 50 Years of Age
• Brief Summary: This Phase 1 clinical trial will evaluate the safety and immunogenicity of an intranasal vaccine candidate, a recombinant, live-attenuated, bovine/human parainfluenza virus vector vaccine expressing the 6-P prefusion-stabilized version of the SARS-CoV-2 spike protein.

Detailed Description

The proposed study is a single center, open label study to be conducted at the Johns Hopkins Bloomberg School of Public Health, Center for Immunization Research (CIR) in Baltimore, MD. Participants will be followed for 12 months after the first immunization, so the duration of study participation will be 12 months after receipt of initial vaccination.

Approximately 30 subjects will be enrolled in a rolling fashion to receive 2 doses of the B/HPIV3/S-6P vaccine intranasally 56 days apart. Participants who are enrolled and receive the first vaccine dose will not be replaced, even in the event they are ineligible or unable to take the second dose.

Up to 60 subjects will be consented to ensure that 30 subjects are eligible for enrollment and vaccination. Volunteers will be enrolled in a staggered fashion:

Group 1: Up to 5 volunteers will be enrolled and vaccinated. Group 2: Up to 10 volunteers will be enrolled and vaccinated. Group 3: The remaining volunteers (up to 15) will be enrolled and vaccinated.

• Study Type: Interventional
• Study Phase: Phase 1

Study Design

Allocation: N/A
Intervention Model: Sequential Assignment
Intervention Model Description:

Recombinant, Live-Attenuated, Bovine/Human Parainfluenza Virus Vector Vaccine Expressing the 6P-Prefusion-Stabilized Version of the SARS-CoV-2 Spike Protein, Administered in two Sequential Doses as Nasal Spray to Adults 18 to 50 Years of Age

Masking: None (Open Label)
Primary Purpose: Prevention

• Condition: SARS-CoV-2 Infection

Intervention

Biological: B/HPIV3/S-6P

Intranasal vaccine.

Study Arms

Experimental: Intranasal Vaccine

B/HPIV3/S-6P vaccine intranasally given in 2 doses, 56 days apart.

Intervention: Biological: B/HPIV3/S-6P

• Publications *: Not Provided

Recruitment Information

• Recruitment Status: Recruiting
• Estimated Enrollment (submitted: August 30, 2023): 30
• Original Estimated Enrollment: Same as current
• Estimated Study Completion Date: June 2025
• Estimated Primary Completion Date: November 2024 (Final data collection date for primary outcome measure)

Eligibility Criteria

Inclusion Criteria:

1. Nonpregnant adults between 18 years and 50 years of age, inclusive.
2. General good health, without significant medical illness, physical examination findings, or significant laboratory abnormalities as determined by the investigator.
3. Demonstrates comprehension of the protocol procedures and knowledge of the trial by passing a written comprehension examination (passing grade > 70%).
4. Available for the duration of the trial.
5. Willingness to participate in the study and cooperate with the study procedures as evidenced by signing the informed consent document.

6. Persons of childbearing potential must have used effective birth control methods for at least one month prior to vaccination, and agree to continue with 'per label/fully effective use' for the chosen method for the duration of the study (30 days prior to first vaccination until 12 months after first vaccination), from amongst these:

   • pharmacologic/hormonal contraceptives, including oral, parenteral, subcutaneous, and transcutaneous delivery;
   • condoms or diaphragm with spermicide;
   • intrauterine device;
   • absolute abstinence from heterosexual intercourse as a matter of normal preferred lifestyle;
   • or must be surgically sterile or have documented menopause, having had no menses at all for at least one full year.

   All persons of childbearing potential must provide samples for urine and serum pregnancy testing prior to enrollment and prior to vaccination. Pregnancy risk assessment and pregnancy prevention counseling will occur at each point of contact.

7. Willingness to refrain from blood donation during participation in the study and for at least 1 year after receiving the second dose of vaccine.
8. Willingness to refrain from receiving vaccines or other investigational products during the first 90 days of the study after enrollment.

Exclusion Criteria:

1. Pregnancy as determined by a positive human choriogonadotropin (ßHCG) test in a person of reproductive capacity.
2. Currently breastfeeding.
3. Evidence of clinically significant neurologic, cardiac, pulmonary, hepatic, rheumatologic, autoimmune, or renal disease by history, physical examination, electrocardiogram (EKG), and/or laboratory studies.
4. Behavioral or cognitive impairment or psychiatric disease that in the opinion of the investigator affects the ability of the subject to understand and cooperate with the study protocol.
5. A history of asthma within the past 5 years, or a current diagnosis of asthma or reactive airway disease associated with exercise, seasonal hay fever or allergic rhinitis.
6. Presence of any febrile illness or symptoms suggestive of a respiratory infection within 2 weeks prior to inoculation.
7. Use of systemic or nasal steroid preparations or immunosuppressive drugs within 30 days prior to vaccination. Topical steroid preparations are permitted.
8. Inhaled bronchodilator or inhaled steroid use within the last year or use after upper respiratory tract infections within the last 5 years.
9. Current or past (in the last 4 weeks) use of intranasal medications (including steroids, decongestants, or hormonal medications), or planning to use them within 28 days of study vaccination.
10. Evidence of current alcohol or illicit drug abuse or addiction.
11. Other condition that in the opinion of the investigator would jeopardize the safety or rights of a subject participating in the trial or would render the subject unable to comply with the protocol.
12. Positive ELISA and confirmatory tests for human immunodeficiency virus (HIV).
13. Positive ELISA and confirmatory test (e.g., recombinant immunoblot assay) for hepatitis C virus (HCV).
14. Positive hepatitis B virus surface antigen (HBsAg) by ELISA.
15. Known immunodeficiency syndrome.
16. Receipt of a live vaccine within 4 weeks or a killed vaccine within 2 weeks prior to receipt of the study vaccine or planned receipt within 90 days after vaccination. (Exception, influenza vaccine may be obtained more than 2 weeks after or prior to the study vaccine).
17. History of a surgical splenectomy.
18. Receipt of blood or blood-derived products (including immunoglobulin [Ig]) within 6 months prior to study inoculation.
19. Has participated in another investigational study involving any investigational product within 30 days, or 5 half-lives, whichever is longer, before the first vaccine administration.
20. Body mass index (BMI) < 18.5 or >40.
21. Any significant abnormality of the nose or nasopharynx, including recurrent epistaxis and including nasal or sinus surgery.
22. History of Bell's palsy.
23. Has a confirmed SARS-CoV-2 infection or COVID-19 vaccine/booster within 16 weeks prior to enrollment.
24. Unwillingness to have nasopharyngeal (NP) or blood samples saved for future respiratory virus research.
25. Pulmonary function test (PFT) testing for FVC, FEV1, FEV1% and PEF of <80 or incentive spirometer value < normal parameters based on gender, age, and height.

Sex/Gender

• Sexes Eligible for Study: All

• Ages: 18 Years to 50 Years (Adult)
• Accepts Healthy Volunteers: Yes

Contacts

Contact: Kawsar Talaat, MD; 410-336-9164; ktalaat@jhu.edu

• Listed Location Countries: United States

Administrative Information

• NCT Number: NCT06026514
• Other Study ID Numbers: CIR 355
• Has Data Monitoring Committee: Yes

U.S. FDA-regulated Product

• Studies a U.S. FDA-regulated Drug Product: Yes
• Studies a U.S. FDA-regulated Device Product: No

• IPD Sharing Statement: Not Provided
• Current Responsible Party: National Institute of Allergy and Infectious Diseases (NIAID)
• Original Responsible Party: Same as current
• Current Study Sponsor: National Institute of Allergy and Infectious Diseases (NIAID)
• Original Study Sponsor: Same as current

Collaborators

• Johns Hopkins Bloomberg School of Public Health
• National Institutes of Health (NIH)

• Investigators: Not Provided
• PRS Account: National Institute of Allergy and Infectious Diseases (NIAID)
• Verification Date: November 2023